| dc.description.abstract | Aims: This study aims to evaluate the in vitro anti-inflammatory and antibacterial potential of
aqueous and various organic solvent (methanol, dichloromethane, and hexane) extracts of Mirabilis
jalapa L. tuberous roots.
Methods: Heat-induced egg albumin denaturation inhibition assay and hypotonicity-induced human
red blood cell (HRBC) membrane stabilization methods were used to evaluate the anti-inflammatory
activity in vitro. Both disc diffusion and well diffusion methods were utilized to evaluate the
antibacterial potential in vitro, using three common wound pathogens. Namely, Escherichia coli
(ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), and Staphylococcus aureus (ATCC
25923).
Results: In the heat-induced egg albumin denaturation inhibition assay, the methanolic extract of
M. jalapa exhibited the highest potency (IC50 = 137.9 µg/ml) of anti-inflammatory activity while in the
HRBC membrane stabilization method, the aqueous extract of M. jalapa exhibited the highest
potency (IC50 = 197.4 µg/ml) of anti-inflammatory activity. There was no significant antibacterial
activity shown by all four extracts. However, some inhibitory zones were observed in the well
diffusion method against S. aureus. The highest inhibitory zone of 15.33 ± 0.33 mm was expressed
by the dichloromethane extract, with a concentration of 400 mg/mL against S. aureus, followed by
the hexane extract with an inhibitory zone of 14.00 ± 2.08 mm at the same concentration. The
dichloromethane extract showed an inhibitory zone of 11.00 ± 0.58 mm at a 200 mg/mL
concentration.
Conclusion: This study demonstrates that the tuberous roots of M. jalapa have significant anti inflammatory properties but no significant antibacterial properties against the selected pathog | en_US |
