Investigation Of In-Vitro Antiurolithiatic Activity Of Passiflora Foetida And Stachytarpheta Indica On Experimentally Prepared Calcium Oxalate Crystals.

Abstract

Abstract:-Urolithiasis or the formation of urinary stones is a major non communicable disease that affects the urinary system. Among the different types of renal stones CaC2O4 are the overwhelming. Antiurolithiatic activity is the inhibition of formation of urinary stones. There is a high demand for herbal medicines to treat urolithiasis due to unavailability of a successful drug devoid of complications. Passiflora foetida (Udahalu) & Stachytarpheta indica (Balunakuta) plants are used in the treatment of kidney stones in traditional medicine. This study was aimed to investigate in-vitro antiurolithiatic activity of these plants on experimentally prepared CaC2O4 crystals. The crude plant extracts were prepared by boiling the dried plant material in water followed by evaporation of the solvents via rotary evaporation. A concentration series of aqueous extract of the plants were prepared with negative and positive (cystone tablets) controls. Antiurolithiatic activity was evaluated using the UV/visible spectrophotometry by measuring optical density of each samples having different concentrations of the plant extracts at 620 nm. The mean absorbance of P.foetida extract at 0.125 mg/mL, 0.25 mg/mL, 0.5 mg/mL, 1 mg/mL, 2 mg/mL, 4 mg/mL concentrations were 0.045, 0.062, 0.105, 0.216, 0.1393, 0.623 respectively. The mean absorbance of S. indica extract at 0.125 mg/mL, 0.25 mg/mL, 0.5 mg/mL, 1 mg/mL, 2 mg/mL, 4 mg/mL concentrations were 0.180, 0.260, 0.201, 0.959, 0.862, 0.492 respectively. There is a concentration dependent increase in the mean absorbance for the samples. The results reveal that both extracts exhibit antiurolithiatic activity and the highest activity is exhibited by S. indica.