| dc.description.abstract | Antibiotic resistance, global health risks, and absurd
consequences of anti-inflammatory drugs are
significant complications. As an alternative plant can be
used. Jeffreycia zeylanica an endemic plant in Sri Lanka
was selected to evaluate antibacterial and anti inflammatory activities, because of its ethnomedical
value and economical. J. zeylanica leaves were air dried, then macerated and plant extracts were
prepared using vacuum evaporation. Plant extracts
were made using aqueous, methanol,
dichloromethane, and hexane as solvents. To detect
antibacterial activity Agar well diffusion and Disc
diffusion methods were used. To assess anti inflammatory activity egg albumin denaturation and
Human Red Blood Cell (HRBC) membrane stabilization
methods were used. The antibacterial activity of the
plant extracts was evaluated against Staphylococcus
aureus, Pseudomonas aeruginosa, and Escherichia coli.
In both methods, plant extracts effectively inhibited
the activity of S. aureus. In the agar well diffusion
method, methanolic extract indicated the highest
inhibition zone of 29.33±0.33 mm and high
effectiveness with EC50 of 39.05 mg/ml. In the disc
diffusion method, dichloromethane extract indicated
the highest zone of 14.66±0.33 mm, and the most
effective activity was indicated by methanolic extract
with an EC50 of 200 mg/ml. Both methods used
Gentamicin (40mg/mL, 10µg) as the positive control. In
the protein denaturation method, hexane extract
indicated the best potential activity with IC50 of 154.9
µg/ml. In the HRBC method, the highest potency was
indicated by dichloromethane extract with IC50 of 154.0
µg/ml. Diclofenac sodium was the reference drug to
evaluate the anti-inflammatory activity. The above
results indicate that plant J. zeylanica has potential
antibacterial and anti-inflammatory activit | en_US |
