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    Evaluation of Synergistic Gastroprotective Activity of Desmodium triflorum Linn. and Pogostemon heyneanus Benth. Using In-Vitro Methods

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    Date
    2022
    Author
    Dias, NTB
    Jayasuriya, WJABN
    Herath, HMDR
    Arawwawala, LDAM
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    Abstract
    Desmodium triflorum Linn. and Pogostemon heyneanus Benth. are common terrestrial plants found in tropical countries. The gastroprotective activities of these individual plants have been investigated separately in previous studies. This study aimed to evaluate the synergistic in-vitro gastroprotective activity of ‘combinations’ of aqueous extracts (AEs) of D. triflorum whole plant and P. heyneanus leaves. AEs of the plants were prepared by refluxing. Thin layer chromatograms for individual plant extracts and combined extracts were obtained after solvent-extracting the AE with dichloromethane. Gastroprotective activities of different combination ratios of the combined extract and the large and small molecular fractions (10000 Da molecular weight cut-off) of the best combination were evaluated in-vitro. A commercially available antacid formulation was used as the positive control, whereas distilled water was used as the negative control. Thin Layer Chromatograms of different combinations of AEs resulted in dissimilar profiles due to the spots with dissimilar Rf values. Different ratios of the combined AEs and, both small (<10000 Da MWC) and large molecular fractions exerted significant (p<0.05) gastroprotection compared to the negative control, based on in-vitro assays of; acidneutralising effect, neutralising capacity by Fordtran’s method and duration of consistent neutralisation by Vatier’s artificial stomach (modified) model. The combination ratio of 3:1 (D. triflorum: P. heyneanus) demonstrated the best activity with acid-neutralising effect assay (4.94±0.00 end pH) and duration of consistent neutralisation (403.43±4.63 s), whereas the positive control resulted in 6.51±0.01 end pH and 450.78±2.87 s in respective assays. Hence, the study concludes that combined AE of D. triflorum whole plant and P. heyneanus leaves exhibit synergistic gastroprotection where the molecules with different molecular weights are responsible for the activity.
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    http://ir.kdu.ac.lk/handle/345/6066
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    • Allied Health Sciences [59]

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