| dc.description.abstract | Dengue has been considered endemic to Sri Lanka since 1989 with regular dengue epidemics. We
sought to identify the dengue strains and the circulating serotypes from July 2019 to December
2021. 299 consenting patients presenting with febrile illness were recruited from the medical
wards at Colombo North Teaching Hospital (CNTH), National Institute for Infectious Diseases
(NIID), and University Hospital- Kotelawala Defence University (UH-KDU) of the western
province. All samples were tested by Reverse transcriptase-polymerase chain reactions (RT-PCR)
and/or real-time-PCR tests to determine dengue positivity and serotype. Sequencing (NGS) was
carried out on samples with adequate viral load (Ct ≤ 22) followed by phylogenetic analysis using
the Geneious software. The testing showed that 51% were positive for the dengue virus. The
serotyping showed that 12% of the positives were DENV-1, 45% DENV-2, 40% DENV-3, and 3%
DENV-4 respectively. Phylogenetic analysis of sequenced samples showed two genotypically
distinct variants of the DENV-3 as well as DENV-1. One DENV-3 variant was similar to those
reported in 2017/18 in Sri Lanka while the other was similar to the one reported in India, in
2016/18. One DENV-1 variant was similar to the 2018 strain found in Sri Lanka while the other
was similar to the variant reported in China in 2014/16. Phylogenetic analysis of DENV-2
sequences showed one cluster of a similar genotype reported in 2017/18. DENV4 is yet to be
sequenced. Our data suggest that during the study period multiple strains have been circulating
in the western province even though no serious epidemic was reported. | en_US |
