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    • Volume 01, Issue 01, 2017
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    • KDU Journal of Multidisciplinary Studies
    • Volume 01, Issue 01, 2017
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    Removal of Microcystin-Lr using Cellular Extracts of Bacillus Cereus

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    KJMS-FGS_p7-p15.pdf (214.8Kb)
    Date
    2017
    Author
    Idroos, Sumaiya F
    Manage, Pathmalal M
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    Abstract
    Microcystin –LR (MC-LR) is considered to be the most dominant type of cyanobacterial toxins present in water bodies. The present study focuses on using cellular extracts of Bacillus cereus in removal of MC-LR in water. Bacterial cell extracts were prepared using overnight grown fresh cultures of B. cereus which was previously recorded as a potent MC-LR degrading bacterium. Bacterial cell disruption was performed by bead beating on a micro-mini bead beater. Cell debris was removed by centrifugation at 13000 rpm, 20 min. Subsequently, a series of concentrations of cellular extract (100%, 75%, 50% and 25%) was prepared. These cell extracts were separately incubated at 280C with 100µg ml-1 of MC-LR for a period of 4 days. 1ml aliquots were removed at 24 hour intervals for four days and frozen at (-20) 0C. Then the frozen samples were freeze-dried and subjected to Photo diode array- High Performance Liquid Chromatography (PDA-HPLC) analysis to detect the remaining MC-LR concentrations of the samples. At the end of fourth day, 81.1 µgml-1 of MC-LR was removed when 100% of cell extract was used. When 75 % of cell extract was used, 77.6 µgml-1 of MC-LR removal was evident at the end of fourth day, whereas when 50 % and 25% of cell extract were used only 40.7 µgml-1 and 25.7 µgml-1 of MC-LR removal was detected respectively. The results of the present study indicate that bacterial cell extracts of B. cereus has the ability to remove MC-LR by an enzyme mediated mechanism.
    URI
    http://ir.kdu.ac.lk/handle/345/3071
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    • Volume 01, Issue 01, 2017 [11]

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