| dc.description.abstract | Antibiotic resistance, global health risks, and absurd consequences of anti-inflammatory
drugs are significant complications. As an alternative plant can be used. Jeffreycia
zeylanica an endemic plant in Sri Lanka was selected to evaluate antibacterial and anti-
inflammatory activities, because of its ethnomedical value and economical. J. zeylanica
leaves were air-dried, then macerated and plant extracts were prepared using vacuum
evaporation. Plant extracts were made using aqueous, methanol, dichloromethane, and
hexane as solvents. To detect antibacterial activity Agar well di usion and Disc di usion
methods were used. To assess anti-in ammatory activity egg albumin denaturation and
Human Red Blood Cell (HRBC) membrane stabilization methods were used. The antibacterial
activity of the plant extracts was evaluated against Staphylococcus aureus, Pseudomonas
aeruginosa, and Escherichia coli. In both methods, plant extracts effectively inhibited the
activity of S. aureus. In the agar well diffusion method, methanolic extract indicated
the highest inhibition zone of 29.33 0.33 mm and high effectiveness with EC50 of 39.05
mg/ml. In the disc diffusion method, dichloromethane extract indicated the highest zone
of 14.66 0.33 mm, and the most effective activity was indicated by methanolic extract
with an EC50 of 200 mg/ml. Both methods used Gentamicin (40mg/mL, 10
g) as the
positive control. In the protein denaturation method, hexane extract indicated the best
potential activity with IC50of 154.9
g/ml. In the HRBC method, the highest potency was
indicated by dichloromethane extract with IC50 of 154.0
g/ml. Diclofenac sodium was
the reference drug to evaluate the anti-inflammatory activity. The above results indicate
that plant J. zeylanica has potential antibacterial and anti-inflammatory activity. | en_US |
