| dc.description.abstract | Biophytum reinwardtii, Trachyspermum roxburghianum, and Cyanthillium cinereum are
traditional herbs in Ayurvedic medicine known for treating hypertension, fatty liver, and
obesity. Abnormal cholesterol levels are considered as a contributing factor for these
conditions. However, the anti-cholesterol properties of these herbs, which are highly
valued in Ayurvedic medicine are yet to be explored. This research aimed to evaluate the
anti-cholesterol activity of di erent plant extracts and analyze qualitative and quantitative
phytochemicals. Plant extraction was carried out by maceration using three di erent
solvents: n-hexane, ethyl acetate, and methanol. The resulting extracts were subjected
to an in vitro cholesterol reduction assay. Qualitative and quantitative phytochemical
analysis of the above three plants were performed exclusively on the methanolic extracts,
which exhibited the highest anti-cholesterol activity. All three plants exhibited signi cant
anti -cholesterol activity compared to the standard Simvastatin, with the methanolic
extracts showing the highest IC50 values. Speci cally, C. cinereum demonstrated the
highest anti-cholesterol capacity, with an IC50 value of 17.48 mg/mL. B. reinwardtii and
T. roxburghianum exhibited anti-cholesterol activity with IC50 values of 30.42 mg/mL
and 27.83 mg/mL, respectively. Phytochemical analysis revealed the presence of phenols,
saponins, alkaloids, tannins, avonoids, and steroids in all methanolic extracts. Notably,
the methanolic extract of C. cinereum displayed the highest total avonoid content (8.23%
w/w) and total saponin content (9.18% w/w), while B. reinwardtii had the highest total
alkaloid content (6.6% w/w). These ndings highlight the potential of the methanol extract
of C. cinereum as a cholesterol-lowering drug. | en_US |
