| dc.description.abstract | Clinicians and scientists are on the lookout for the development of safer, sustainable
alternatives for topical corticosteroids in treating in ammatory skin diseases due to the
potential complications associated with their long-term use. Although both honey and
Aloe vera have been individually studied for their anti-in ammatory action, there is only
limited research on the speci c combination of Sri Lankan bee’s (SLB) honey with Aloe
vera. In this study, the anti-in ammatory activities of the individual components and
combinations are evaluated. A heat-induced albumin denaturation assay was used. Data
analysis was carried out using the GraphPad Prism 9 software. At 5000
g/mL, the
highest of the concentration range used in this study. The aqueous solutions of SLB honey
and crude Aloe vera exhibited moderate anti-in ammatory activities of 8.19 0.08% and
8.14 0.93%, respectively. Lyophilization of the crude Aloe vera gel e ectively increased
its activity up to 77.53 0.16%, at the same concentration. The combinations of SLB
honey with crude Aloe vera, and SLB honey with lyophilized Aloe vera demonstrated a
percentage inhibition of albumin denaturation of 12.59 2.4% and 86.51 0.2% respectively.
Thus, when combined, SLB honey with Aloe vera show higher activity compared to their
individual counterparts. The concentrations at which optimal activity was observed
were selected for formulation. Accordingly, three novel topical anti-in ammatory gels
were formulated with 0.125%, 0.25%, and 0.5% SLB honey and lyophilized Aloe vera (1:1)
which exhibited relatively high activities of 71.57 0.48%, 76.56 0.24%, and 81.38 0.21%
respectively. The organoleptic properties, pH, and spreadability of the gels were observed
to be stable over a 60-days period at 4 C, room temperature, and 40 C. With further
optimization and testing, the gels would show a promising potential to be developed into
a clinically useful product. | en_US |
