dc.description.abstract | Foodborne illnesses are a significant global concern and Doner kebab, a popular street
food, which grilled meatloaf renowned for its diverse nutritional composition which is
prone to microbial contamination. The present study aimed to determine the microbiological quality of street food, Doner kebab in the Colombo district, Sri Lanka. One hundred
fifty (n = 150) Doner kebab samples were purchased randomly from three regions of the
Colombo district including Aluthkade, Kibulawala, and Kailiza. The microorganisms
were isolated and identified via conventional standard biochemical keys including Indole,
MR-VP, Citrate, Motility, TSI, Urease, Bile, Oxidase, and Gram stain. The analysis was
obtained from MicrosoftExcell 2010 and SPSS V23.0. Eight pathogenic bacterial species
namely; Klebsiella spp. (13%), Pseudomonas spp. (07%), Escherichia coli (20%), Enterobacter
aerogenes (20%), Salmonella spp. (20%), Shigella spp. (07%), Vibrio spp. (07%) and
Protease spp. (07%) were identified. Among lactose-fermented microorganisms Escherichia
coli (37%), Enterobacter aerogenes (25%), and Klebsiella spp. (38%) were detected while
among non-lactose fermented microorganisms, highest percentage 44% of Salmonella spp.
following equally 14% of Shigella spp., Proteus spp. Vibrio spp. and Pseudomonas spp. were
detected. All three regions were contaminated with at least three types of microorganisms.
Compared to the Aluthkade, Kalubowila (P = 0.020, p < 0.05), Aluthkade, Kalisa (P
= 0.003, p < 0.05) and Kalubowila, Kalisa (P = 0.000, p < 0.05) showed a significant
different in contamination. The highest significant Escherichia coli (40%) contamination
was detected in Aluthkade while Enterobacter aerogenes (40%) and Salmonella spp. (40%)
in Kailiza (p < 0.05) All three regions showed the highest pathogenic contamination thus,
implementing measures to reduce the contaminations are important. | |