| dc.description.abstract | Mikania cordata (Burm. f.) Robinson is a herbaceous vine belonging to the family
Asteraceae. Traditionally it is mainly used for cuts and wounds. This study aims to
evaluate wound healing ability and chemical constituents in M. cordata leaves
responsible for wound healing. Leaf extracts were prepared by either crushing or
sonicating with different solvents. Scratch assay was performed to analyse cell
migration in vitro using ethanolic extract of M. cordata leaves and C6 glioma cells.
Cells were grown in culture dishes to create a wound as a scratch. Cells were
incubated in ethanolic extracts having 10, 100, 500, and 1000 μg/ml concentrations,
and cell movement was observed using a fluorescence microscope. Live cell images
were recorded and analysed at different time intervals (0th hour, 6-, 12- and 24-
hour) to quantify the cell migration with time. The 1000 μg/ml ethanolic extract of
M. cordata leaves was found to have a positive effect on cell migration with a 100%
wound closure rate over a 24-hour incubation period. Next, thin-layer
chromatography (TLC) and column chromatography were carried out to separate
the chemical constituents. TLC chromatograms showed that sonicated leaf extracts
had fewer chlorophyll traces than crushed extracts. The high amount of plant
pigment traces could conceal the bioactive chemical constituents. Hence, the study
was continued with leaf extracts prepared by sonication. Leaf powder sonicated in
DCM had more chemical compounds than the other extracts. Future studies have to
be carried out on DCM extract and scratch assay to build the correlation between
leaf extract and wound healing. | en_US |
