| dc.description.abstract | Serum creatinine determination is crucialin
assessing renal functions. Jaffe reaction is used
world-wide in serum creatinine estimation. It has
been reported that bilirubin affects Jaffe reaction
causing underestimation. However, Creatinase
method has little interference but is comparatively
more expensive. The objective of this study
was to compare the creatinine concentrations of
serum with different bilirubin levels using Jaffe
reaction and Creatinase method. Three serum
pools were placed in an infant phototherapy
incubator for 18 hours to photolysis the bilirubin.
Serum creatinine concentration of each pool was
measured by Creatinase method and the values
were 0.51, 2.41 and 7.33mg/dL. Each serum pool
was then spiked with eleven different bilirubin
concentrations.The bilirubin concentrations of
the spiked serum were 0, 3, 6, 9, 12, 15, 18, 21, 24,
27 and 30mg/dL. The total bilirubin, creatinine
by Jaffe reaction and Creatinase method were
measured by Indiko TM clinical and specialty
chemistry system. Results were analyzed by
linear regression analysis. According to the
Creatinase method, there was no significant
underestimation in serum with 0.51mg/dL of
creatinine but Jaffe reaction showed a significant
underestimationfrom bilirubin concentration
of 7.18mg/dL. In serum with 2.41mg/dL of
creatinine, Creatinase method and Jaffe reaction
were given significant underestimations by
bilirubin concentrations of 9.05 and 5.64mg/dL,
respectively. In serum of 7.33mg/dL creatinine,
significant underestimations were given from
bilirubin levels of 3.6 and 8.18mg/dL by Creatinase
method and Jaffe reaction, respectively. According
to the present study, Creatinase method is reliable
in estimation of serum creatinine of ≤0.51mg/dL. | en_US |
