| dc.description.abstract | Chnoospora minima (Herring) is a brown algae
that belongs to Family Scytosiphonaceae. The
current study investigated the total phenolic (TP),
total flavonoid contents (TFC), antioxidants and
anti-amylase activity of the methanolic extract of
C. minima. The sample was extracted with 70%
methanol and polysaccharides were separated.
The resulting polyphenol portion was obtained
for analysis. TP, TF contents were determined
using standard methods. Antioxidant assays
were conducted using ferric reducing antioxidant
power (FRAP), ferrous iron chelating activity,
DPPH and ABTS radical scavenging activity.
Anti-diabetic potential was assessed using
anti-amylase assay. TP and TF contents were
2950.72±87.09 (μg gallic acid equivalent/g
of sample) and 39.36±7.81 (μg quercetin
equivalents/g of sample) respectively. The free
radical scavenging activity of the C. minima
revealed that the reduction of DPPH occurred
in a concentration-dependent manner with high
reductions occurring at the highest concentrations.
The DPPH and ABTS radical scavenging activity
of C. minima was 959.45±13.91 and 5734.22±120.11
μg trolox equivalent for 1 g sample respectvely.
The IC50 of ABTS and DPPH radical scavenging
activity was 74.34±1.56 μg/ml and 452.42±6.51
μg/ml when compared to the standard trolox
(IC50:8.68±0.06 μg/ml). The ferric reducing antioxidant
power of C. minima was 293.32±59.66 μg
Trolox per 1g of sample. The ferrous iron chelating
activity of the methanolic extract of C. minima
was 278.22±5.62 μg EDTA per 1 g of sample. The
anti-amylase assay showed that, C. minima
inhibited the activity of α-amylase with IC50 value
of 17.93±0.19 μg/ml and the percentage inhibition
varied from 89.58 to 3.69. According to the results,
methanolic extract of C. minima possessed high
antioxidant and α-amylase inhibition activity,
thus increasing its potential therapeutic property. | en_US |
