Evaluation of Random Donor Platelets Produced from Buffy Coat Stored for 24 hrs at Ambient Temperature. A Better Alternative to 8 hr Limitation

Abstract

Introduction-Whole blood derived platelets are made from platelet rich plasma (PRP) method or buffy coat method. In India majority of random donor platelets are prepared by PRP method. However, Buffy coat method offers the advantage of less platelet activation and fewer WBC contamination. Presently in India RDPs are prepared within 8 hour of whole blood collection, whereas, in Europe this time limit is up to 24 hours. Aim-Our aim was to evaluate the platelet count, WBC contamination, platelet CD 62 P expression, and biochemical parameters of RDPs prepared from buffy coat within 8 hours and within 24 hours of collection. Materials and method-We prepared 40 units of RDP by the buffy coat method from whole blood stored at room temperature within 8 hours of collection (Fresh BC),& another 40 units from buffy coat stored at 22°C for less than 24 hours (Stored BC). We analyzed the platelet counts, CD62P expression, WBC counts, glucose levels, pH, PO2, PCO2 in both the groups of RDPs, 24 hours after respective preparation. Results-The platelet count from stored BC was higher in fresh BC. CD 62P expression was low in stored BC compared to fresh BC. There were no differences pH, pO2, pCO2 and glucose levels between fresh BC and stored BC. WBC contamination was more in fresh BC. Conclusion-Our study stored BC contained higher platelet counts, less WBC contamination and less platelet activation. We conclude that RDP prepared from stored BC is the better method for RDP production