Journal Articles
http://ir.kdu.ac.lk/handle/345/3889
2024-03-28T09:10:03ZRole Played by Biochemical and Hematological Parameters in the Prediction of Cardiovascular Risk
http://ir.kdu.ac.lk/handle/345/7299
Role Played by Biochemical and Hematological Parameters in the Prediction of Cardiovascular Risk
Ranaweera, CB; Nuwanthika, WKT; Welivitigoda, DIK; Wijesinghe, N; Kottahachchi, DU
The term “cardiovascular diseases” (CVDs) refers to any disease affecting the heart or blood vessels. CVDs are the most common cause of death worldwide and the most responsible reason for 10% of deaths in the early 20th century. These CVDs can emerge as myocardial infarction or ischemic heart disease, stroke, and congestive heart failure. For diagnosing CVDs, physical, radiological investigations, and laboratory investigations for cardiac enzymes and lipid profile are used. In clinical practices, cardiovascular risk prediction models are very important in the identification, prevention, and staging of the severity of CVDs. Framingham Risk Score-coronary heart disease, Framingham Risk Score-cardiovascular disease, QRESEARCH-cardiovascular risk algorithm, Joint British Society risk-calculator-3, WHO/ISH CVD risk prediction charts, and Atherosclerotic Cardiovascular Disease risk-estimator are some of the available CVD risk estimators. However, many of these estimators can be used only to evaluate individuals more than 40 years and to assess the risk for 10-years. Therefore, new risk estimators are needed to overcome the deficiencies of the available risk estimators. Research have been done in novel directions; on ratios of routinely performing hematological and biochemical laboratory parameters such as Neutrophil to Lymphocyte Ratio (NLR), Platelet to Lymphocyte Ratio (PLR), and Aspartate aminotransferase to Alanine aminotransferase ratio (AST/ALT). The outcomes indicate a relationship between the above-said ratios and the risk for CVDs. Conclusion:On such grounds, this review describes the importance of developing a CVD risk estimator by amalgamating some of the biochemical and hematological parameters, including NLR, PLR, and AST/ALT ratios, aiming to overcome the existing shortcomings.
2024-03-11T00:00:00ZA Study of the Associations of Platelet-Indices and Lipid Profile Parameters of the Individuals With no Previous History of Cardiovascular Diseases
http://ir.kdu.ac.lk/handle/345/7149
A Study of the Associations of Platelet-Indices and Lipid Profile Parameters of the Individuals With no Previous History of Cardiovascular Diseases
Ranaweera, CB; Senadeera, SPNN; Kottahachchi, DU; Wakwella, TN; Welivitigoda, DIK; Arachchi, CPU; Mauri, KDI; Wijesinghe, Namal
Platelet-Indices (PIs) are markers of platelet activation, which relates to the platelet’s prolif-
eration kinetics and morphology. Platelets play a key-role in the aetiology of cardiovascular
diseases (CVDs), including atherosclerosis and its outcomes. This study was carried out to
investigate whether the people who do not have any previous history of CVDs possess any reliable
correlations in between PIs and lipid profile parameters. The study included 60 volunteers from
the UH-KDU health and administrative staff; ages 40-70 years, males (80%) & females (20%).
Full blood count (FBC) parameters of the participants were performed by automated analyser
Sysmex-XP-100 and cross-checked by manual differential counts, while the lipid profile param-
eters were carried-out manually. The statistical analysis was carried-out by IBM-SPSS V26.
The data were first examined for normalization. Pearson and Spearman’s correlation bivariate
analyses were applied when the data showed normal and did not show normal respectively, for
the two sets of parameters to establish correlations in-between them. In the Spearman bivariate
analysis, the PIs, mean platelet volume (MPV) and platelet large cell ratio (PLCR) showed
significant (p<0.05) weak to moderate correlations only with low-density-lipoprotein (LDL)
(r=0.292; r=0.301). Furthermore, there were a considerable number of significant (p<0.05)
reliable correlations in-between the platelet indices. It has been revealed that the platelet indices
have a positive correlation with CVDs and, also reactive & large platelets present in patients with
dyslipidemia lead to increased risk for CVDs. Moreover, the chronic inflammation and increased
oxidative stress which is prominent in CVDs are accompanied with elevated levels of LDL of an
individual. In our study, some of the PIs had weak to moderate positive correlations with LDL
although the participants had no previous CVD history. Therefore, we suggest increasing the
number of participants in order to examine the similar findings and further to monitor them for
risk of the CVDs.
2023-12-06T00:00:00ZIn vitro Anti-Inflammatory Activity of Leaves of Jeffreycia zeylanica Using the Egg Albumin Denaturation Method and Human Red Blood Cell Stabilization Method
http://ir.kdu.ac.lk/handle/345/7126
In vitro Anti-Inflammatory Activity of Leaves of Jeffreycia zeylanica Using the Egg Albumin Denaturation Method and Human Red Blood Cell Stabilization Method
Ranaweera, CB; Senadeera, Nimesha N; Samaraweera, Thumuli; Samaraweera, Thummini
Aims To investigate the in vitro anti-inflammatory activity of aqueous, methanol, dichloromethane (DCM), and hexane extracts of Jeffreycia zeylanica leaves extracts. In vitro protein (egg albumin) denaturation method and in vitro human red blood cell (HRBC) membrane stabilization methods were used to evaluate the anti-inflammatory activity of the leaf extracts.
Methodology Plant leaves were collected, washed, air dried, and obtained crude plant material. Cold maceration was used to extract the plant materials. Aqueous, methanol, DCM, and hexane were used as solvents for maceration. Diclofenac sodium was used as the positive control for the evaluation.
Results In the egg albumin denaturation method, the positive control diclofenac sodium indicated an IC 50 value of 179.2 µg/ml and R2 of 0.9979. Similarly, hexane leaf extract suggested an IC 50 value of 154.9 µg/ml and R2 of 0.9114. P values for all the extracts were P<0.05 indicating that there’s a strong correlation between plant extract concentration and % inhibition of egg albumin denaturation. According to the IC 50 values and R2 value hexane extract indicated the highest potential anti-inflammatory activity. In the HRBC membrane stabilization method, the positive control diclofenac sodium indicated an IC 50 value of 77.05 µg/ml and R2 of 0.9929 similarly DCM leaf extract indicated an IC 50 value of 154.0 µg/ml and R2 of 0.9787. P values for all the extracts were P<0.05 indicating that there’s a strong correlation between plant extracts concentration and % protection of RBC membrane. According to the IC 50 values and R2 value DCM extract indicated the highest potential anti-inflammatory activity.
Conclusion Hexane leaf extract indicated the highest potential anti-inflammatory activity for the egg albumin denaturation method meanwhile DCM leaf extract indicated the highest potential anti-inflammatory activity for the HRBC membrane stabilization method, evidencing that nonpolar or less polar secondary metabolites of the plant leaves can contribute more to the plant’s anti-inflammatory activity.
2023-11-25T00:00:00ZClitoria ternatea-Shifting Paradigms: From Laboratory to Industry
http://ir.kdu.ac.lk/handle/345/6679
Clitoria ternatea-Shifting Paradigms: From Laboratory to Industry
Ranaweera, CB; Chandana, AK
Clitoria ternateacommonly known as Butterfly pea is a standard Ayurvedic medicinal plant used in many parts of south Asian countries. Traditional medicinal plants are a great alternative to find new treatments and for the development of novel antimicrobials to combat many diseases. In Ayurveda and traditional and folk medicine in several countries, decoction and extracts made from C. ternatea are recommended to be used for various medical treatments. C. ternateaextracts claimed to possess antibacterial, antiviral, and antifungal properties, which had been supported and validated by many in vitroand in vivoexperiments. However, biologically active compound/s isolation and development novel compounds still remain in its infancy. Despite its enormous potential health benefits, only a single commercial product managed to reach industrial level production. C. ternateacyclotide studies are also limited despite the fact that it the fastest known natural ligase discovered to date. These cyclotides are rapid peptide ligators and has been the focus of many recent studies on peptide ligation and cyclization for biotechnological applications. In this mini summary we have tried to point out innate unique biological properties of C. ternatea and suggested few future studies, more specifically on C. ternatea cyclotides development against bacterial heat shock proteins (Hsp 100) for novel antimicrobial discovery and development.
2021-12-03T00:00:00Z